The American Journal of Surgery
Volume 194, Issue 5 , Pages 618-622 , November 2007

Cytoskeletal signaling by way of α-actinin-1 mediates ERK1/2 activation by repetitive deformation in human Caco2 intestinal epithelial cells

Presented at the 31st Annual Surgical Symposium of the Association of VA Surgeons, Little Rock, AR, May 10–12, 2007

  • David H. Craig, B.S.

      Affiliations

    • Department of Surgery, John D. Dingell VA Medical Center and Wayne State University, Detroit, MI 48201, USA
    • ,
  • Jianhu Zhang, Ph.D.

      Affiliations

    • Department of Surgery, John D. Dingell VA Medical Center and Wayne State University, Detroit, MI 48201, USA
    • ,
  • Marc D. Basson, M.D., Ph.D., M.B.A.

      Affiliations

    • Department of Surgery, John D. Dingell VA Medical Center and Wayne State University, Detroit, MI 48201, USA
    • Department of Anesthesiology, John D. Dingell VA Medical Center and Wayne State University, Detroit, MI 48201, USA
    • Department of Anatomy and Cell Biology, Wayne State University, Detroit, MI 48201, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-313-576-3598; fax: +1-313-576-1002.

Received 14 May 2007 ,Revised 30 July 2007

References 

  1. Ashida N, Takechi H, Kita T, et al. Vortex-mediated mechanical stress induces integrin-dependent cell adhesion mediated by inositol 1,4,5-trisphosphate-sensitive Ca2+ release in THP-1 cells. J Biol Chem. 2003;278:9327–9331
  2. Haier J, Nicolson GL. Tumor cell adhesion under hydrodynamic conditions of fluid flow. APMIS. 2001;109:241–262
  3. Shin HY, Gerritsen ME, Bizios R. Regulation of endothelial cell proliferation and apoptosis by cyclic pressure. Ann Biomed Eng. 2002;30:297–304
  4. Basson MD. Paradigms for mechanical signal transduction in the intestinal epithelium (Category: molecular, cell, and developmental biology). Digestion. 2003;68:217–225
  5. Zhang J, Li W, Sanders MA, et al. Regulation of the intestinal epithelial response to cyclic strain by extracellular matrix proteins. FASEB J. 2003;17:926–928
  6. Basson MD, Li GD, Hong F, et al. Amplitude-dependent modulation of brush border enzymes and proliferation by cyclic strain in human intestinal Caco-2 monolayers. J Cell Physiol. 1996;168:476–488
  7. Basson MD, Turowski G, Emenaker NJ. Regulation of human (Caco-2) intestinal epithelial cell differentiation by extracellular matrix proteins. Exp Cell Res. 1996;225:301–305
  8. Spencer AU, Sun X, El-Sawaf M, et al. Enterogenesis in a clinically feasible model of mechanical small-bowel lengthening. Surgery. 2006;140:212–220
  9. Zittel TT, Meile T, Huge A, et al. Preoperative intraluminal application of capsaicin increases postoperative gastric and colonic motility in rats. J Gastrointest Surg. 2001;5:503–513
  10. Li W, Duzgun A, Sumpio BE, et al. Integrin and FAK-mediated MAPK activation is required for cyclic strain mitogenic effects in Caco-2 cells. Am J Physiol Gastrointest Liver Physiol. 2001;280:G75–G87
  11. Chaturvedi LS, Marsh HM, Shang X, et al. Repetitive deformation activates focal adhesion kinase and ERK mitogenic signals in human Caco-2 intestinal epithelial cells through Src and Rac1. J Biol Chem. 2007;282:14–28
  12. Basson MD, Coppola CP. Repetitive deformation and pressure activate small bowel and colonic mucosal tyrosine kinase activity in vivo. Metabolism. 2002;51:1525–1527
  13. Atance J, Yost MJ, Carver W. Influence of the extracellular matrix on the regulation of cardiac fibroblast behavior by mechanical stretch. J Cell Physiol. 2004;200:377–386
  14. Yang F, Li YH. Roles of integrins and cytoskeleton in cellular mechanotransduction. Space Med Med Eng (Beijing). 2002;15:309–312
  15. Huynh TT, Iaccarino G, Davies MG, et al. External support modulates G protein expression and receptor coupling in experimental vein grafts. Surgery. 1999;126:127–134
  16. Ghazi A, Berrier C, Ajouz B, et al. Mechanosensitive ion channels and their mode of activation. Biochimie. 1998;80:357–362
  17. Katsumi A, Orr AW, Tzima E, et al. Integrins in mechanotransduction. J Biol Chem. 2004;279:12001–12004
  18. Ingber DE. Tensegrity: the architectural basis of cellular mechanotransduction. Annu Rev Physiol. 1997;59:575–599
  19. Basson MD, Modlin IM, Madri JA. Human enterocyte (Caco-2) migration is modulated in vitro by extracellular matrix composition and epidermal growth factor. J Clin Invest. 1992;90:15–23
  20. Rajfur Z, Roy P, Otey C, et al. Dissecting the link between stress fibres and focal adhesions by CALI with EGFP fusion proteins. Nat Cell Biol. 2002;4:286–293
  21. Herreros L, Rodriguez-Fernandez JL, Brown MC, et al. Paxillin localizes to the lymphocyte microtubule organizing center and associates with the microtubule cytoskeleton. J Biol Chem. 2000;275:26436–26440
  22. Pavalko FM, Otey CA, Simon KO, et al. Alpha-actinin: a direct link between actin and integrins. Biochem Soc Trans. 1991;19:1065–1069
  23. Honda K, Yamada T, Endo R, et al. Actinin-4, a novel actin-bundling protein associated with cell motility and cancer invasion. J Cell Biol. 1998;140:1383–1393
  24. Zhang J, Owen CR, Sanders MA, et al. The motogenic effects of cyclic mechanical strain on intestinal epithelial monolayer wound closure are matrix dependent. Gastroenterology. 2006;131:1179–1189
  25. Coppolino MG, Dedhar S. Bi-directional signal transduction by integrin receptors. Int J Biochem Cell Biol. 2000;32:171–188
  26. Ingber DE. Tensegrity II (How structural networks influence cellular information processing networks). J Cell Sci. 2003;116:1397–1408
  27. Thamilselvan V, Basson MD. The role of the cytoskeleton in differentially regulating pressure-mediated effects on malignant colonocyte focal adhesion signaling and cell adhesion. Carcinogenesis. 2005;26:1687–1697
  28. Leinweber BD, Leavis PC, Grabarek Z, et al. Extracellular regulated kinase (ERK) interaction with actin and the calponin homology (CH) domain of actin-binding proteins. Biochem J. 1999;344:117–123
  29. Tatsumi Y, Cho YY, He Z, et al. Involvement of the paxillin pathway in JB6 Cl41 cell transformation. Cancer Res. 2006;66:5968–5974
  30. Conway WC, Van der Voort van Zyp J, Thamilselvan V, et al. Paxillin modulates squamous cancer cell adhesion and is important in pressure-augmented adhesion. J Cell Biochem. 2006;98:1507–1516
  31. van Zyp JV, Conway WC, Craig DH, et al. Extracellular pressure stimulates tumor cell adhesion in vitro by paxillin activation. Cancer Biol Ther. 2006;5:1169–1178

PII: S0002-9610(07)00631-9

doi: 10.1016/j.amjsurg.2007.08.001

The American Journal of Surgery
Volume 194, Issue 5 , Pages 618-622 , November 2007

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