The American Journal of Surgery
Volume 198, Issue 2 , Pages 203-209 , August 2009

Eighteen months clinical experience with the GeneSearch breast lymph node assay

  • Isabelle Veys, M.D.

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Virginie Durbecq, Ph.D.

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Samira Majjaj, Ph.D.

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Jean Christophe Schobbens, M.D.

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Danielle Noterman, M.D.

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Nicolas Sirtaine, M.D.

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Maria Dolores Martin Martinez, M.D.

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Dina Hertens, M.D.

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Francesco Feoli, M.D.

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Pierre Bourgeois, M.D., Ph.D.

      Affiliations

    • Department of Nuclear Medicine, Jules Bordet Institute, Université Libre de Bruxelles, Brussels, Belgium
  • ,
  • Jean-Marie Nogaret, M.D.

      Affiliations

    • Department of Surgery, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
  • ,
  • Denis Larsimont, M.D., Ph.D.

      Affiliations

    • Department of Pathology, Jules Bordet Institute, Université Libre de Bruxelles, Belgium
    • Corresponding Author InformationCorresponding author. Tel.: +32-2-541-31-15; fax: +32-2-541-32-81

Received 16 May 2008 ,Revised 29 September 2008

References 

  1. Giuliano AE, Dale PS, Turner RR, et al. Improved axillary staging of breast cancer with sentinel lymphadenectomy. Ann Surg. 1995;222:394–399
  2. Fisher ER, Anderson S, Redmond C, et al. Pathologic findings from the national surgical adjuvant breast project protocol B-06: 10-year pathologic and clinical prognostic discriminates. Cancer. 1993;71:2507–2514
  3. Ivens D, Hoe AL, Podd TJ, et al. Assessment of morbidity from complete axillary dissection. Br J Cancer. 1992;66:136–138
  4. Recht A, Houlihan MJ. Axillary lymph nodes and breast cancer: a review. Cancer. 1995;76:1491–1512
  5. Giuliano AE, Haigh PI, Brennan MB, et al. Prospective observational study of sentinel lymphadenectomy without further axillary dissection in patients with sentinel node-negative breast cancer. J Clin Oncol. 2000;18:2553–2559
  6. Giuliano AE, Jones RC, Brennan M, et al. Sentinel lymphadenectomy in breast cancer. J Clin Oncol. 1997;15:2345–2350
  7. Veronesi U, Paganelli G, Galimberti V, et al. Sentinel-node biopsy to avoid axillary dissection in breast cancer with clinically negative lymph-nodes. Lancet. 1997;349:1864–1867
  8. Veronesi U, Paganelli G, Viale G, et al. Sentinel lymph node biopsy and axillary dissection in breast cancer: results in a large series. J Natl Cancer Inst. 1999;91:368–373
  9. Reitsamer R, Peintinger F, Prokop E, et al. 200 Sentinel lymph node biopsies without axillary lymph node dissection: no axillary recurrences after a 3-year follow-up. Br J Cancer. 2004;90:1551–1554
  10. Schwartz GF, Giuliano AE, Veronesi U. Proceeding of the consensus conference on the role of sentinel lymph node biopsy in carcinoma of the breast, April 19–22, 2001, Philadelphia, Pennsylvania. Cancer. 2002;94:2542–2551
  11. Roberts CA, Beitsch PD, Litz CE, et al. Interpretive disparity among pathologists in breast sentinel lymph node evaluation. Am J Surg. 2003;186:324–329
  12. Hunt JL, Baloch ZW, LiVolsi VA. Sentinel lymph node evaluation for tumor metastasis. Semin Diagn Pathol. 2002;19:263–277
  13. Liu LH, Siziopikou KP, Gabram S, et al. Evaluation of axillary sentinel lymph node biopsy by immunohistochemistry and multilevel sectioning in patients with breast carcinoma. Arch Pathol Lab Med. 2000;124:1670–1673
  14. Pargaonkar AS, Beissner RS, Snyder S, et al. Evaluation of immunohistochemistry and multiple-level sectioning in sentinel lymph nodes from patients with breast cancer. Arch Pathol Lab Med. 2003;127:701–705
  15. van Diest PJ, Torrenga H, Borgstein PJ, et al. Reliability of intraoperative frozen section and imprint cytological investigation of sentinel lymph nodes in breast cancer. Histopathology. 1999;35:14–18
  16. Turner RR, Hansen NM, Stern SL, et al. Intraoperative examination of the sentinel lymph node for breast carcinoma staging. Am J Clin Pathol. 1999;112:627–634
  17. Motomura K, Inaji H, Komoike Y, et al. Intraoperative sentinel node examination by imprint cytology and frozen sectioning during breast surgery. Br J Surg. 2000;87:597–601
  18. Schoenfeld A, Luqmani Y, Smith D, et al. Detection of breast cancer micrometastases in axillary lymph nodes by using polymerase chain reaction. Cancer Res. 1994;54:2986–2990
  19. Noguchi S, Aihara T, Motomura K, et al. Detection of breast cancer micrometastases in axillary lymph nodes by means of reverse transcriptase-polymerase chain reaction: comparison between MUC1 mRNA and keratin 19 mRNA amplification. Am J Pathol. 1996;148:649–656
  20. Aihara T, Fujiwara Y, Ooka M, et al. Mammaglobin B as a novel marker for detection of breast cancer micrometastases in axillary lymph nodes by reverse transcription-polymerase chain reaction. Breast Cancer Res Treat. 1999;58:137–140
  21. Inokuchi M, Ninomiya I, Tsugawa K, et al. Quantitative evaluation of metastases in axillary lymph nodes of breast cancer. Br J Cancer. 2003;89:1750–1756
  22. Manzotti M, Dell'orto P, Maisonneuve P, et al. Reverse transcription-polymerase chain reaction assay for multiple mRNA markers in the detection of breast cancer metastases in sentinel lymph nodes. Int J Cancer. 2001;95:307–312
  23. Nissan A, Pager D, Roystacher M, et al. Multimarker RT-PCR assay for the detection of minimal residual disease in sentinel lymph nodes of breast cancer patients. Br J Cancer. 2006;94:681–685
  24. Hughes SJ, Xi L, Raja S, et al. A rapid, fully automated, molecular-based assay accurately analyzes sentinel lymph nodes for the presence of metastatic breast cancer. Ann Surg. 2006;243:389–398
  25. Gillanders WE, Mikhitarian K, Hebert R, et al. Molecular detection of micrometastatic breast cancer in histopathology-negative axillary lymph nodes correlates with traditional predictors of prognosis: an interim analysis of a prospective multi-institutional cohort study. Ann Surg. 2004;239:828–837
  26. Gould VE, Bloom KJ, Franke WW, et al. Increased numbers of cytokeratin-positive interstitial reticulum cells (CIRC) in reactive, inflammatory and neoplastic lymphadenopathies: hyperplasia or induced expression?. Virchows Arch. 1995;425:617–629
  27. Martin Martinez L, Veys I, Majjaj S, et al. Clinical validation of a molecular assay for intra-operative detection of metastases in breast sentinel lymph nodes. Eur J Surg Oncol, in Press.
  28. Lyman GH, Giuliano AE, Somerfield MR, et al. American Society of Clinical Oncology guideline recommendations for sentinel lymph node biopsy in early-stage breast cancer. J Clin Oncol. 2005;23:7703–7720
  29. Bourgeois P, Nogaret JM, Veys I, et al. How hot is the pathologically positive sentinel lymph node in breast cancer patients?. Nucl Med Commun. 2003;24:513–518
  30. Blumencranz P, Whitworth PW, Deck K, et al. Sentinel node staging for breast cancer: intraoperative molecular pathology overcomes conventional histologic sampling errors. Am J Surg. 2007;194:426–432
  31. Julian T, Blumencranz P, Deck K. Novel intra-operative molecular test for sentinel lymph node metastases in patients with early stage beast cancer. J Clin Oncol. 2008;26:3338–3345
  32. Krag DN, Anderson SJ, Julian TB, et al. Technical outcomes of sentinel-lymph-node resection and conventional axillary-lymph-node dissection in patients with clinically node-negative breast cancer: results from the NSABP B-32 randomized phase III trial. Lancet Oncol. 2007;8:881–888
  33. Van Rijk MC, Peterse JL, Nieweg OE, et al. Additional axillary metastases and stage migration in breast cancer patients with micrometastases or submicrometastases in sentinel lymph nodes. Cancer. 2006;107:467–471
  34. Calhoun KE, Hansen NM, Turner RR, et al. Nonsentinel node metastases in breast cancer patients with isolated tumor cells in the sentinel node: implications for completion axillary node dissection. Am J Surg. 2005;190:588–591
  35. Viale G, Dell'orto P, Biasi MO, et al. Comparative evaluation of the extensive histopathologic examination and real-time reverse-transcription-polymerase chain reaction assay for mammaglobin and cytokeratin 19 on axillary sentinel lymph nodes of breast carcinoma patients. Ann Surg. 2008;247:136–142
  36. Kurosumi M, Takei H. Significance and problems of histopathological examination and utility of real-time reverse transcriptase-polymerase chain reaction method for detection of sentinel lymph node metastasis in breast cancer. Breast Cancer. 2007;14:342–349
  37. Yared MA, Middleton LP, Smith TL, et al. Recommendation for sentinel lymph node processing in breast cancer. Am J Surg Pathol. 2002;26:377–382

PII: S0002-9610(08)00824-6

doi: 10.1016/j.amjsurg.2008.09.012

The American Journal of Surgery
Volume 198, Issue 2 , Pages 203-209 , August 2009