Overexpression of LAPTM4B promotes growth of gallbladder carcinoma cells in vitro
Abstract
Background
The overexpression of LAPTM4B-35 in gallbladder carcinoma (GBC) and its clinicopathologic and prognostic significance have been previously shown. Thus, this gene may play a role in the growth of GBC cells.
Methods
The pcDNA3-AE containing the complete open reading frame of LAPTM4B (lysosome-associated protein transmembrane-4β) and mock (pcDNA3) plasmids were transiently transfected into GBC-SD cells. Cell proliferation, cell cycle distribution, and protein expression were evaluated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium assay, flow cytometry, and Western blot, respectively.
Results
Cells transfected with pcDNA3-AE revealed accelerated proliferation, less serum dependence, and significant cell cycle progression compared with cells transfected with mock plasmid and parent cells. These phenotypes were accompanied by upregulated expression of C-myc, c-Fos, c-Jun, cyclin D1, and cyclin E and downregulated expression of P16 and P-27.
Conclusions
LAPTM4B overexpression promotes the growth of GBC cells in vitro by regulating the expression levels of some proliferation-associated proteins. Therefore, the LAPTM4B gene might be used as a novel therapeutic target of GBC.
Keywords: LAPTM4B, Transfection, Proliferation, Gallbladder carcinoma
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This work was supported by a grant for the municipal key discipline of Beijing, China (HK100230446).
PII: S0002-9610(09)00509-1
doi:10.1016/j.amjsurg.2009.03.031
© 2010 Elsevier Inc. All rights reserved.
