The American Journal of Surgery
Volume 199, Issue 4 , Pages 542-548 , April 2010

Cyclosporine A–protection against microvascular hyperpermeability is calcineurin independent

  • Ed W. Childs, M.D.

      Affiliations

    • Department of Surgery, Texas A&M Health Science Center College of Medicine and Scott & White Memorial Hospital, 2401 South 31st St., Temple, TX 76508, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-254-724-5638; fax: +1-254-724-7912
    • ,
  • Binu Tharakan, Ph.D.

      Affiliations

    • Department of Surgery, Texas A&M Health Science Center College of Medicine and Scott & White Memorial Hospital, 2401 South 31st St., Temple, TX 76508, USA
    • ,
  • Suliat Nurudeen, B.S.

      Affiliations

    • Department of Surgery, Texas A&M Health Science Center College of Medicine and Scott & White Memorial Hospital, 2401 South 31st St., Temple, TX 76508, USA
    • ,
  • Thomas L. Delmas, B.S.

      Affiliations

    • Department of Surgery, Texas A&M Health Science Center College of Medicine and Scott & White Memorial Hospital, 2401 South 31st St., Temple, TX 76508, USA
    • ,
  • Joseph Hellman, B.S.

      Affiliations

    • Department of Surgery, Texas A&M Health Science Center College of Medicine and Scott & White Memorial Hospital, 2401 South 31st St., Temple, TX 76508, USA
    • ,
  • Tasheika Christie, B.S.

      Affiliations

    • Undergraduate Medical Academy, Prairie View A&M University, Prairie View, TX, USA
    • ,
  • Felicia A. Hunter, B.S.

      Affiliations

    • Department of Surgery, Texas A&M Health Science Center College of Medicine and Scott & White Memorial Hospital, 2401 South 31st St., Temple, TX 76508, USA
    • ,
  • W. Roy Smythe, M.D.

      Affiliations

    • Department of Surgery, Texas A&M Health Science Center College of Medicine and Scott & White Memorial Hospital, 2401 South 31st St., Temple, TX 76508, USA

Received 1 July 2009 ,Revised 20 November 2009

References 

  1. Baluk P, Hirata A, Thurston G, et al. Endothelial gaps: time course of formation and closure in inflamed venules of rats. Am J Physiol. 1997;272:155–170
  2. Childs EW, Udobi KF, Hunter FA, et al. Evidence of transcellular albumin transport after hemorrhagic shock. Shock. 2005;23:565–570
  3. Childs EW, Tharakan B, Hunter FA, et al. Apoptotic signaling induces hyperpermeability following hemorrhagic shock. Am J Physiol Heart Circ Physiol. 2007;292:H3179–H3189
  4. Tharakan B, Holder-Haynes JG, Hunter FA, et al. Cyclosporine A prevents vascular hyperpermeability after hemorrhagic shock by inhibiting apoptotic signaling. J Trauma. 2009;66:1033–1039
  5. Tharakan B, Corprew R, Hunter FA, et al. 17beta-estradiol mediates protection against microvascular endothelial cell hyperpermeability. Am J Surg. 2009;197:147–154
  6. Kim R, Emi M, Tanabe K. Caspase-dependent and -independent cell death pathways after DNA damage. Oncol Rep. 2005;14:595–599
  7. Herren B, Levkau B, Raines EW, et al. Cleavage of beta-catenin and plakoglobin and shedding of VE-cadherin during endothelial apoptosis: evidence for a role for caspases and metalloproteinases. Mol Biol Cell. 1998;9:1589–1601
  8. Bannerman DD, Sathyamoorthy M, Goldblum SE. Bacterial lipopolysaccharide disrupts endothelial monolayer integrity and survival signaling events through caspase cleavage of adherens junction proteins. J Biol Chem. 1998;273:35371–35380
  9. Childs EW, Tharakan B, Byrge N, et al. Angiopoietin-1 inhibits intrinsic apoptotic signaling and vascular hyperpermeability following hemorrhagic shock. Am J Physiol Heart Circ Physiol. 2008;294:H2285–H2295
  10. Walter DH, Haendeler J, Galle J, et al. Cyclosporin A inhibits apoptosis of human endothelial cells by preventing release of cytochrome c from mitochondria. Circulation. 1998;98:1153–1157
  11. Crabtree GR. Calcium, calcineurin, and the control of transcription. J Biol Chem. 2001;276:2313–2316
  12. Squadrito F, Altavilla D, Squadrito G, et al. Protective effects of cyclosporin-A in splanchnic artery occlusion shock. Br J Pharmacol. 2000;130:339–344
  13. Woolley SM, Farivar AS, Naidu BV, et al. Endotracheal calcineurin inhibition ameliorates injury in an experimental model of lung ischemia-reperfusion. J Thorac Cardiovasc Surg. 2004;127:376–384
  14. Armstrong JS, Yang H, Duan W, et al. Cytochrome bc(1) regulates the mitochondrial permeability transition by two distinct pathways. J Biol Chem. 2004;279:50420–50428
  15. Santana LF, Chase EG, Votaw VS, et al. Functional coupling of calcineurin and protein kinase A in mouse ventricular myocytes. J Physiol. 2002;544:57–69
  16. Bradford MM. A Rapid and Sensitive method for the quantitation of microgram quantities of protein utilizing the protein-dye binding. Anal. Biochem. 1976;72:248–254
  17. Halestrap AP, Connern CP, Griffiths EJ, et al. Cyclosporin A binding to mitochondrial cyclophilin inhibits the permeability transition pore and protects hearts from ischaemia/reperfusion injury. Mol Cell Biochem. 1997;174:167–172
  18. Kroemer G. Mitochondrial control of apoptosis: an introduction. Biochem Biophys Res Commun. 2003;304:433–435
  19. McStay GP, Clarke SJ, Halestrap AP. Role of critical thiol groups on the matrix surface of the adenine nucleotide translocase in the mechanism of the mitochondrial permeability transition pore. Biochem J. 2002;367:541–548
  20. Halestrap AP, Brennerb C. The adenine nucleotide translocase: a central component of the mitochondrial permeability transition pore and key player in cell death. Curr Med Chem. 2003;10:1507–1525
  21. Garrido C, Galluzi L, Brunet M, et al. Mechanism of cytochrome c release from mitochondria. Cell Death Diff. 2006;13:1423–1433
  22. Gregorc U, Ivanova S, Thomas M, et al. hDLG/SAP97, a member of the MAGUK protein family, is a novel caspase target during cell-cell detachment in apoptosis. Biol Chem. 2005;386:705–710
  23. Hunter I, McGregor D, Robins SP. Caspase-dependent cleavage of cadherins and catenins during osteoblast apoptosis. J Bone Miner Res. 2001;16:466–477
  24. Cattelino A, Liebner S, Gallini R, et al. The conditional inactivation of the beta-catenin gene in endothelial cells causes a defective vascular pattern and increased vascular fragility. J Cell Biol. 2003;162:1111–1122
  25. Cervello M, Giannitrapani L, La Rosa M, et al. Induction of apoptosis by the proteasome inhibitor MG132 in human HCC cells: possible correlation with specific caspase-dependent cleavage of beta-catenin and inhibition of beta-catenin-mediated transactivation. Int J Mol Med. 2004;13:741–748
  26. Van de Craen M, Berx G, Van den Brande I, et al. Proteolytic cleavage of beta-catenin by caspases: an in vitro analysis. FEBS Lett. 1999;458:167–170
  27. Nakamoto K, Kuratsu J, Ozawa M. Beta-catenin cleavage in non-apoptotic cells with reduced cell adhesion activity. Int J Mol Med. 2005;15:973–979
  28. Steinhusen U, Badock V, Bauer A, et al. Apoptosis-induced cleavage of beta-catenin by caspase-3 results in proteolytic fragments with reduced transactivation potential. J Biol Chem. 2000;275:16345–16353

PII: S0002-9610(09)00760-0

doi: 10.1016/j.amjsurg.2009.11.002

The American Journal of Surgery
Volume 199, Issue 4 , Pages 542-548 , April 2010

Article Tools

* Image Usage & Resolution