The American Journal of Surgery
Volume 200, Issue 2 , Pages 283-290 , August 2010

Nuclear factor kappa B–dependent gene transcription in cholecystokinin- and tumor necrosis factor-α–stimulated isolated acinar cells is regulated by p38 mitogen-activated protein kinase

Abstract presented at the annual meeting of the Association of VA Surgeons, Boston, MA, April 19, 2009.

  • Deborah E. Williard, B.S.Ch.E.

      Affiliations

    • Surgical Services, Iowa City Veterans Affairs Medical Center, Iowa City, IA, USA
    • Department of Surgery, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, USA
  • ,
  • Erik Twait

      Affiliations

    • Surgical Services, Iowa City Veterans Affairs Medical Center, Iowa City, IA, USA
    • Department of Surgery, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, USA
  • ,
  • Zuobiao Yuan, M.D., Ph.D.

      Affiliations

    • Surgical Services, Iowa City Veterans Affairs Medical Center, Iowa City, IA, USA
    • Department of Surgery, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, USA
  • ,
  • A. Brent Carter, M.D.

      Affiliations

    • Surgical Services, Iowa City Veterans Affairs Medical Center, Iowa City, IA, USA
    • Department of Internal Medicine, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, USA
  • ,
  • Isaac Samuel, M.D., F.R.C.S., F.A.C.S.

      Affiliations

    • Surgical Services, Iowa City Veterans Affairs Medical Center, Iowa City, IA, USA
    • Department of Surgery, Roy J. and Lucille A. Carver College of Medicine, University of Iowa, Iowa City, IA, USA
    • Corresponding Author InformationCorresponding author. Tel.: +1-319-356-7359; fax: +1-319-356-8378

Received 15 July 2009 ,Revised 20 November 2009

References 

  1. Norman J. The role of cytokines in the pathogenesis of acute pancreatitis. Am J Surg. 1998;175:76–83
  2. Samuel I, Zaheer S, Zaheer A. Bile-pancreatic juice exclusion increases p38MAPK activation and TNF-alpha production in ligation-induced acute pancreatitis in rats. Pancreatology. 2005;5:20–26
  3. Carter AB, Knudtson KL, Monick MM, et al. The p38 mitogen-activated protein kinase is required for NF-kappaB-dependent gene expression (The role of TATA-binding protein (TBP)). J Biol Chem. 1999;274:30858–30863
  4. Carter AB, Hunninghake GW. A constitutive active MEK→ERK pathway negatively regulates NF-kappa B-dependent gene expression by modulating TATA-binding protein phosphorylation. J Biol Chem. 2000;275:27858–27864
  5. Chew J, Biswas S, Shreeram S, et al. WIP1 phosphatase is a negative regulator of NF-kappaB signalling. Nat Cell Biol. 2009;
  6. Samuel I, Tephly L, Williard DE, et al. Enteral exclusion increases map kinase activation and cytokine production in a model of gallstone pancreatitis. Pancreatology. 2008;8:6–14
  7. Sanlioglu S, Williams CM, Samavati L, et al. Lipopolysaccharide induces Rac1-dependent reactive oxygen species formation and coordinates tumor necrosis factor-alpha secretion through IKK regulation of NF-kappa B. J Biol Chem. 2001;276:30188–30198
  8. Bi Y, Page SL, Williams JA. Rho and Rac promote acinar morphological changes, actin reorganization, and amylase secretion. Am J Physiol Gastrointest Liver Physiol. 2005;289:G561–G570
  9. Kaplan R, Zaheer A, Jaye M, et al. Molecular cloning and expression of biologically active human glia maturation factor-beta. J Neurochem. 1991;57:483–490
  10. Ghosh S, Karin M. Missing pieces in the NF-kappaB puzzle. Cell. 2002;109(Suppl):S81–S96
  11. Zhong H, SuYang H, Erdjument-Bromage H, et al. The transcriptional activity of NF-kappaB is regulated by the IkappaB-associated PKAc subunit through a cyclic AMP-independent mechanism. Cell. 1997;89:413–424
  12. Zhong H, Voll RE, Ghosh S. Phosphorylation of NF-kappa B p65 by PKA stimulates transcriptional activity by promoting a novel bivalent interaction with the coactivator CBP/p300. Mol Cell. 1998;1:661–671
  13. Anrather J, Csizmadia V, Soares MP, et al. Regulation of NF-kappaB RelA phosphorylation and transcriptional activity by p21(Ras) and protein kinase Czeta in primary endothelial cells. J Biol Chem. 1999;274:13594–13603
  14. Sakurai H, Chiba H, Miyoshi H, et al. Kinases phosphorylate NF-kappaB p65 subunit on serine 536 in the transactivation domain. J Biol Chem. 1999;274:30353–30356
  15. Madrid LV, Wang CY, Guttridge DC, et al. Akt suppresses apoptosis by stimulating the transactivation potential of the RelA/p65 subunit of NF-kappaB. Mol Cell Biol. 2000;20:1626–1638
  16. Jang MK, Goo YH, Sohn YC, et al. 2+/Calmodulin-dependent protein kinase IV stimulates nuclear factor-kappa B transactivation via phosphorylation of the p65 subunit. J Biol Chem. 2001;276:20005–20010
  17. Schafer C, Williams JA. Stress kinases and heat shock proteins in the pancreas: possible roles in normal function and disease. J Gastroenterol. 2000;35:1–9
  18. Yang J, Murphy C, Denham W, et al. Evidence of a central role for p38 map kinase induction of tumor necrosis factor alpha in pancreatitis-associated pulmonary injury. Surgery. 1999;126:216–222
  19. Samuel I, Zaheer S, Nelson JJ, et al. CCK-A receptor induction and P 38 and NF-kappaB activation in acute pancreatitis. Pancreatology. 2004;4:49–56
  20. Fleischer F, Dabew R, Goke B, et al. Stress kinase inhibition modulates acute experimental pancreatitis. World J Gastroenterol. 2001;7:259–265
  21. Murr MM, Yang J, Fier A, et al. Regulation of Kupffer cell TNF gene expression during experimental acute pancreatitis: the role of p38-MAPK, ERK1/2, SAPK/JNK, and NF-kappa B. J Gastrointest Surg. 2003;7:20–25
  22. Samuel I, Zaheer A, Fisher RA. In vitro evidence for role of ERK, p38, and JNK in exocrine pancreatic cytokine production. J Gastrointest Surg. 2006;10:1376–1383
  23. Morel C, Ibarz G, Oiry C, et al. Cross-interactions of two p38 mitogen-activated protein (MAP) kinase inhibitors and two cholecystokinin (CCK) receptor antagonists with the CCK1 receptor and p38 MAP kinase. J Biol Chem. 2005;280:21384–21393
  24. Meyerholz DK, Williard DE, Grittmann AM, et al. Murine pancreatic duct ligation induces stress kinase activation, acute pancreatitis, and acute lung injury. Am J Surg. 2008;196:675–682
  25. Chen X, Ji B, Han B, et al. NF-kappaB activation in pancreas induces pancreatic and systemic inflammatory response. Gastroenterology. 2002;122:448–457
  26. Leach SD, Modlin IM, Scheele GA, et al. Intracellular activation of digestive zymogens in rat pancreatic acini. J Clin Invest. 1991;87:362–366
  27. Chaudhuri A, Kolodecik TR, Gorelick FS. Effects of increased intracellular cAMP on carbachol-stimulated zymogen activation, secretion, and injury in the pancreatic acinar cell. Am J Physiol Gastrointest Liver Physiol. 2005;288:G235–G243
  28. Husain SZ, Grant WM, Gorelick FS, et al. Caerulein-induced intracellular pancreatic zymogen activation is dependent on calcineurin. Am J Physiol Gastrointest Liver Physiol. 2007;292:G1594–G1599
  29. Singh VP, Saluja AK, Bhagat L, et al. Phosphatidylinositol 3-kinase-dependent activation of trypsinogen modulates the severity of acute pancreatitis. J Clin Invest. 2001;108:1387–1395
  30. Wagner AC, Williams JA. Calcium induces rapid changes in protein phosphorylation in permeabilized pancreatic acini. Pancreas. 1995;11:236–240
  31. Li C, Chen X, Williams JA. Regulation of CCK-induced amylase release by PKC-delta in rat pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol. 2004;287:G764–G771
  32. Satoh A, Gukovskaya AS, Edderkaoui M, et al. Tumor necrosis factor-alpha mediates pancreatitis responses in acinar cells via protein kinase C and proline-rich tyrosine kinase 2. Gastroenterology. 2005;129:639–651
  33. Satoh A, Gukovskaya AS, Nieto JM, et al. PKC-delta and -epsilon regulate NF-kappaB activation induced by cholecystokinin and TNF-alpha in pancreatic acinar cells. Am J Physiol Gastrointest Liver Physiol. 2004;287:G582–G591

PII: S0002-9610(10)00070-X

doi: 10.1016/j.amjsurg.2009.12.004

The American Journal of Surgery
Volume 200, Issue 2 , Pages 283-290 , August 2010