The American Journal of Surgery
Volume 170, Issue 2 , Pages 126-130, August 1995

In vivo platelet deposition on polytetrafluoroethylene coated with fibrin glue containing fibroblast growth factor 1 and heparin in a canine model☆☆☆

  • Claire Gosselin, MD

      Affiliations

    • Department of Surgery, Loyola University Medical Center, Maywood, Illinois, USA
    • ,
  • Dewei Ren, MD

      Affiliations

    • Department of Surgery, Loyola University Medical Center, Maywood, Illinois, USA
    • ,
  • Joan Ellinger

      Affiliations

    • Department of Surgery Hines VA Hospital, Hines, Illinois, USA
    • ,
  • Howard P. Greisler, MD

      Affiliations

    • Corresponding Author InformationRequests for reprints should be addressed to Howard P. Greisler, MD, Loyola University Medical Center, Department of Surgery, 2160 South First Avenue, Maywood, Illinois 60153.
    • Department of Surgery, Loyola University Medical Center, Maywood, Illinois, USA
    • Department of Cell Biology, Neurobiology and Anatomy, Loyola University Medical Center, Maywood, Illinois, USA
    • Department of Surgery Hines VA Hospital, Hines, Illinois, USA

Abstract 

Background: We previously reported that the coating of expanded polytetrafluoroethylene (ePTFE) with fibrin glue containing fibroblast growth factor 1 (FGF-1) and heparin accelerates endothelial coverage of grafts implanted into animals. We report here the effect of this surface modification on early platelet deposition.

Materials and methods: Nine dogs received 7-cm ePTFE grafts, 60-μm internodal distance, 4-mm internal diameter, as bilateral aortoiliac implants, one coated (luminal cross section and abluminal surface) with fibrin glue (fibrinogen 32.1 mg/mL, thrombin 0.32 U/mL) containing FGF-1 (11 ng/mL) and heparin (250 U/mL), the other uncoated. After 5, 30, or 120 minutes of circulation with blood containing autologous platelets radiolabeled with indium 111, γ emissions were quantitated on explants and correlated to surface areas measured by computerized planimetry.

Results: Both global and segmental comparisons showed significantly (P <0.05, Student's t-test) less platelet deposition on coated than on uncoated grafts after 120 minutes of circulation, but no difference at 5 and 30 minutes.

Conclusions: In this model, ePTFE coating with fibrin glue containing FGF-1 and heparin shows no adverse effect on early platelet deposition.

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☆ Supported by grant RO1 HL41272 from the National Institutes of Health.Dr. Gosselin is supported by a grant from the R. Samuel McLaughlin Foundation, Toronto, Canada.

☆☆ Presented at the 23rd Annual Meeting of The Society for Clinical Vascular Surgery, Fort Lauderdale, Florida, March 22–26, 1995.

PII: S0002-9610(99)80270-0

The American Journal of Surgery
Volume 170, Issue 2 , Pages 126-130, August 1995

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