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Limiting the rate of reperfusion blood flow has been shown to be beneficial locally
in models of ischemia-reperfusion injury. We investigated the effects of this on eicosanoids
(thromboxane B2, 6-keto-PGF1α, and leukotriene B4), white blood cell activation, and skeletal muscle injury as quantitated by triphenyltetrazolium
chloride and technetium-99m pyrophosphate after ischemia-reperfusion injury in an
isolated gracilis muscle model in 16 anesthetized dogs. One gracilis muscle in each
dog was subjected to 6 hours of ischemia followed by 1 hour of limited reperfusion
and then by a second hour of normal reperfusion. The other muscle was subjected to
6 hours of ischemia followed by 2 hours of normal reperfusion. Six dogs each were
used as normal reperfusion controls (NR) and limited reperfusion controls (LR), with
5 dogs being treated with a thromboxane synthetase inhibitor (LR/TSI) and another
five with a leukotriene inhibitor (LR/LI). LR in all three groups (LR, LR/TSI, and
LR/LI) showed a benefit in skeletal muscle injury as measured by triphenyltetrazolim
chloride and technetium-99m pyrophosphate when compared with NR. However, there was
no significant difference between the groups with LR regarding eicosanoid levels and
white blood cell activation when compared with NR. These results demonstrate that
LR produces benefits by mechanisms other than those dependent upon thromboxane A2, prostacyclin, or white blood cell activation.
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Article info
Footnotes
*This work has been designated the 1990 Peter B. Samuels Prize Essay by a Resident. Dr. Anderson is the recipient of the award.
2Presented at the 18th Annual Meeting of the Society for Clinical Vascular Surgery, Palm Desert, California, March 7–11, 1990.
Identification
Copyright
© 1990 Reed Publishing USA. Published by Elsevier Inc.
