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Research Article| Volume 164, ISSUE 3, P199-204, September 1992

New evidence and new hope concerning endothelial seeding of vascular grafts

  • Author Footnotes
    2 the Plastic Surgery, University of Southern California, Los Angeles, California
    Anthony Sank
    Correspondence
    Requests for reprints should be addressed to Anthony Sank, MD, Department of Surgery, Division of Plastic Surgery, University of Southern California, School of Medicine, 1200 North State Street, Los Angeles, California 90033.
    Footnotes
    2 the Plastic Surgery, University of Southern California, Los Angeles, California
    Affiliations
    Los Angeles, California, USA
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  • Author Footnotes
    1 From the Department of Surgery,University of Southern California, Los Angeles, California
    Khodam Rostami
    Footnotes
    1 From the Department of Surgery,University of Southern California, Los Angeles, California
    Affiliations
    Los Angeles, California, USA
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  • Author Footnotes
    3 Vascular Surgery Divisions, University ofSouthern California, Los Angeles, California
    Fred Weaver
    Footnotes
    3 Vascular Surgery Divisions, University ofSouthern California, Los Angeles, California
    Affiliations
    Los Angeles, California, USA
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  • Author Footnotes
    1 From the Department of Surgery,University of Southern California, Los Angeles, California
    Delia Ertl
    Footnotes
    1 From the Department of Surgery,University of Southern California, Los Angeles, California
    Affiliations
    Los Angeles, California, USA
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  • Author Footnotes
    3 Vascular Surgery Divisions, University ofSouthern California, Los Angeles, California
    Albert Yellin
    Footnotes
    3 Vascular Surgery Divisions, University ofSouthern California, Los Angeles, California
    Affiliations
    Los Angeles, California, USA
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  • Author Footnotes
    1 From the Department of Surgery,University of Southern California, Los Angeles, California
    Marcel Nimni
    Footnotes
    1 From the Department of Surgery,University of Southern California, Los Angeles, California
    Affiliations
    Los Angeles, California, USA
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  • Author Footnotes
    2 the Plastic Surgery, University of Southern California, Los Angeles, California
    ,
    Author Footnotes
    4 Dr. Tuan is supported by Grant 40409 from NIHAR.
    Tai-Lan Tuan
    Footnotes
    2 the Plastic Surgery, University of Southern California, Los Angeles, California
    4 Dr. Tuan is supported by Grant 40409 from NIHAR.
    Affiliations
    Los Angeles, California, USA
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  • Author Footnotes
    2 the Plastic Surgery, University of Southern California, Los Angeles, California
    1 From the Department of Surgery,University of Southern California, Los Angeles, California
    3 Vascular Surgery Divisions, University ofSouthern California, Los Angeles, California
    4 Dr. Tuan is supported by Grant 40409 from NIHAR.
DOI:https://doi.org/10.1016/S0002-9610(05)81070-0
New evidence and new hope concerning endothelial seeding of vascular grafts
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      Endothelial cell (EC) seeding of prosthetic bypass grafts has been promoted as a method of improving graft patency. However, an efficient and reliable method of seeding vascular prostheses with ECs is lacking due to inefficient harvesting of ECs and poor attachment and proliferation of cells on the prosthetic surfaces. To investigate the effect of a commonly used prosthetic surface on EC attachment and proliferation, we measured the attachment and proliferation of ECs on polytetrafluoroethylene (PTFE) grafts uncoated or coated with gelatin, laminin, fibronectin, collagen type I and/or III, or RGD (arginine-glycine-aspartate)-containing peptide. EC attachment and proliferation were both significantly decreased on the untreated PTFE graft surface. Conversely, coating of PTFE with fibronectin, RGD, laminin, or gelatin significantly (p <0.05) improved the attachment of ECs, with the most striking increases occurring with laminin and gelatin. Similarly, all matrix components in this study improved EC proliferation compared with untreated PTFE, with RGD and gelatin producing the most significant improvement.
      PTFE adversely effects EC attachment and proliferation.These properties can be improved by treating PTFE graft surfaces with extracellular matrix components in relatively low concentrations. Future investigations are needed to determine whether there are combinations and concentrations of matrix components that will optimize these cellular functions on vascular prostheses.
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      Article info

      Footnotes

      **This work was supported in part by grants from the Plastic Surgery Educational Foundation, Children's Hospital of Los Angeles, Aesthetic Foundation, and the National Institutes of Health (AR10358)

      *This work was awarded the 1992 Allastair Karmody ResearchAward for Medical Students. Khodam Rostami is the recipient of the award.

      ‡Presented at the 20th Annual Meeting of the Society for Clinical Vascular Surgery, Orlando, Florida, March 25–29, 1992.

      Identification

      DOI: https://doi.org/10.1016/S0002-9610(05)81070-0

      Copyright

      © 1992 Reed Publishing USA. Published by Elsevier Inc.

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