Abstract
Background
Small unilamellar lipid vesicles were used to encapsulate adenosine triphosphate (ATP-vesicles)
for intracellular energy delivery and were tested for diabetic skin wounds in rabbits.
Methods
Diabetes was induced by alloxan. The mean peak blood glucose concentration was 505
mg/dL. One ear was made ischemic and 80 full-thickness wounds were created in 10 animals.
ATP-vesicles or saline was used and healing was compared.
Results
On the non-ischemic ears, mean closure time for ATP-vesicles–treated wounds was 13.7
days versus 16.4 days for saline-treated wounds (P < .05). On the ischemic ears, mean closure time for ATP-vesicles–treated wounds was
15.3 days versus 19.3 days for saline-treated wounds (P < .01). Histological study indicated better healing and re-epithelialization in the
ATP-vesicles–treated wounds.
Conclusions
Intracellular delivery of ATP accelerated the healing process of diabetic skin wounds
on ischemic and non-ischemic rabbit ears. The mechanisms deserve further study but
may be related to improved cellular energy supplies.
Keywords
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Article info
Publication history
Received in revised form:
May 1,
2009
Received:
January 2,
2009
Footnotes
Sufan Chien is the partner of Novera, LLC in Louisville, which plans to further develop and commercialize the ATP-vesicle for wound treatment.
Identification
Copyright
© 2010 Elsevier Inc. Published by Elsevier Inc. All rights reserved.
